Özet:
Lung cancer is the leading cause of cancer-related deaths in males and second after breast cancer in females. Lung cancer etiology is mainly genetic and epigenetic damage caused by smoking. According to recent studies, orientin (Ot) and vicenin-2 (Vc), two water-soluble flavonoids extracted from Indian Holy Basil, can function as antitumor by stopping inducing apoptosis, as well as anti-proliferative and anti-angiogenic. The aim of the study was to investigate the effect of orientin and vicenin-2 on the growth and apoptosis of A549 cells and to study their effect on Sema 3F, VEGF and Np-2.
Material methods: The cytotoxicity of our compounds against A549 (cancer cell line) and CCD-19 and L929 (normal cell lines) was determined by MTT assay and their apoptotic effects were determined by flow cytometry methods. Our compunds effect on expression levels of genes involved in apoptotic and cell proliferation on A549 cells was studied with Real time PCR.
Results: In this study, orientin and vicenim demonstrated anticancer activity against the A549 cell line, a) significantly reduced viability of A549 cells (p <0.001), b) low toxicity in MTT tests on healthy cells c) inducing both late and early apoptosis by flow cytometry experiments has been observed. In oreintin treated cells Bax expressions of apoptosis-related proteins increased, while antiapoptotic Bcl-2 expression decreased, e) orientin up-regulates tumor suppressor gene Sema 3F, which can also block VEGF effect associated with increased cell migration and metastasis. f) orientin has also been shown to significantly down-regulate nueruphilin- 2, which is overexpressed in lung cancer. g) It has also been shown to down-regulate the PERK and ERK genes. These results provided in vitro evidence of orientin‘s and to a lesser ectent vicenin‘s antitumor particularly apoptotic effects. Further preclinical trials on the orientin and the vicenin-2 are needed to further investigate the maximum potential as an anti-cancer agent in lung cancer
